Methodology for indole-3-acetic acid: Sample preparation, extraction, and purification techniques
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چکیده
منابع مشابه
Indole-3-Acetic Acid and Flowering.
Science, a weekly journal, Is published each Friday by the American Association for the Advancement of Science at The Business Press, Incorporated, N. Queen St. and McGovern Ave., Lancaster, P'a. Founded In 1880. it has been since 1900 the official publication of the AAAS. Editorial and Advertising Offices, 1515 Massachusetts Avenue, N.W.. Washington 5, D. C. Telephone, EXecutive 80 or 661. Cab...
متن کاملOxidation of indole-3-acetic acid to oxindole-3-acetic acid by an enzyme preparation from Zea mays.
Indole-3-acetic acid is oxidized to oxindole-3-acetic acid by Zea mays tissue extracts. Shoot, root, and endosperm tissues have enzyme activities of 1 to 10 picomoles per hour per milligram protein. The enzyme is heat labile, is soluble, and requires oxygen for activity. Cofactors of mixed function oxygenase, peroxidase, and intermolecular dioxygenase are not stimulatory to enzymic activity....
متن کاملPurification and Partial Characterization of a Glucan Containing Indole-3-acetic Acid.
The "bound auxin" of Zea mays, first described by Berger and Avery (Amer. J. Bot. 1944; 31: 199-203) has been purified and partially characterized. It is an indole-3-acetic acid-containing, high molecular weight, lipophilic cellulosicglucan. The indole-3-acetic acid is in ester linkage as evidenced by indoleacetamide formation upon ammonolysis. The glucan is of variable chain length and compris...
متن کاملOxindole-3-acetic Acid, an Indole-3-acetic Acid Catabolite in Zea mays.
A prior study (13) from this laboratory showed that oxidation of exogenously applied indole-3-acetic acid (IAA) to oxindole-3-acetic acid (OxIAA) is the major catabolic pathway for IAA in Zea mays endosperm. In this work, we demonstrate that OxIAA is a naturally occurring compound in shoot and endosperm tissue of Z. mays and that the amount of OxIAA in both shoot and endosperm tissue is approxi...
متن کاملIndole-3-acetic acid in microbial and microorganism-plant signaling.
Diverse bacterial species possess the ability to produce the auxin phytohormone indole-3-acetic acid (IAA). Different biosynthesis pathways have been identified and redundancy for IAA biosynthesis is widespread among plant-associated bacteria. Interactions between IAA-producing bacteria and plants lead to diverse outcomes on the plant side, varying from pathogenesis to phyto-stimulation. Review...
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ژورنال
عنوان ژورنال: Hilgardia
سال: 1986
ISSN: 0073-2230
DOI: 10.3733/hilg.v54n06p015